Hamamatsu News 2 / 2017

BIOASTER, a new model for technology innovation in microbiology The BIOASTER Technology Research Institute (TRI) was created in April 2012 by the Institut Pasteur and Lyonbiopole health competitiveness cluster, following the initiative of the French Government through the “Investissements d’Avenir” Program. BIOASTER is leading collaborative projects that bring together academics, start-ups, SMEs and industrial groups, and is developing a unique technological and innovative model in order to overcome technological bottlenecks and explore new avenues applied to micro­ biology in health and diseases. In this context, the Preclinical Models & Imaging Unit tested the Hamamatsu NanoZoomer S60 with its fluorescence module, to evaluate its relevance for bacteria imaging in relation to the study of host-microbiota and microbes-microbes interactions. FISHing of gut microbiota with the NanoZoomer S60 Risson A., Tamellini A., Darnaud M. News 2017 Vol. 2 14 0 50 100 150 200 250 µm 0 10 20 30 40 50 µm 0 20 40 60 80 100 µm Application Report Figure 2: Mouse colon section showing epithelial cell nuclei stained with DAPI (blue) and Lactobacilli distribution within the lumen, detected by the FISH probe LGC0355 labeled with the fluorophore CY5 (red). 20x objective (above), magnification to 40x (middle) and additional numerical zoom to 80x (below). Figure 1: Whole mouse colon section stained with DAPI (blue) showing high level of green autofluorescence from the gut epithelium and lumen, which can be a limitation for imaging samples with green channel (FITC). 20x objective, 4x view. 0 100 200 300 400 500 µm